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PIE Scientific LLC plasma cleaner tergeo-em
Plasma Cleaner Tergeo Em, supplied by PIE Scientific LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tergeo+em+plasma+cleaner/tergeo+plasma+cleaner/pmc12262184-242-8-11
Average 90 stars, based on 1 article reviews
plasma cleaner tergeo-em - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Clinical Proteomics:

Article Title: Two simple ways of making clean nitrogen-doped graphene for TEM studies
Article Snippet: .. Samples were treated with a Tergeo EM Plasma Cleaner from Pie Scientific, which is capable of generating a conductively coupled plasma at a frequency of 13.75 MHz. ..

Article Title: TFIIH kinase CDK7 drives cell proliferation through a common core transcription factor network.
Article Snippet: .. CAK- MAT1Δ219 (2 mg/ ml) was diluted 7× in the buffer containing the 54 μM SY- 5609 and incubated at room temperature for 5 min. SY- 1365 (purchased from Insight Biotech Ltd.) was dissolved in DMSO and diluted to 10 μM in cryo- EM buffer before formation of the covalent complex by incubation with diluted CAK complex for 15 min. For grid preparation, UltrAuFoil 1.2/1.3 300- mesh gold grids (Quantifoil Micro Tools GmbH) were plasma cleaned for 50 s using a Tergeo EM Plasma Cleaner (PIE Scientific). .. Four microliters of sample was applied on the grid and blotted for 1.5 s using a Vitrobot Mark IV (Thermo Fisher Scientific) and then plunge frozen in liquid ethane cooled by liquid nitrogen.

Article Title: Panta Rhei - tuning silver nanostructure evolution with flow and radiolysis in liquid phase STEM
Article Snippet: The platinum electrodes are only biased (using a Gamry Instruments Reference 620) with three pulses of − 5 nA for 10 s each for the experiment shown in Fig. 1(b) for the qualitative assessment and remain unbiased for the quantitative assessment in the rest of the study. .. To facilitate wetting of the nanochannel, the liquid cell microchips were pre-treated in a 1 min H2O remote plasma using a Tergeo EM plasma cleaner from PIE Scientific. .. An aqueous solution of 10 mM AgNO3 was prepared by dissolving 0.167 g of AgNO3 salt (supplied by SigmaAldrich, purity ≥ 99%) in 100 mL of deionized water.

Article Title: Selective peptide bond formation via side chain reactivity and self-assembly of abiotic phosphates.
Article Snippet: .. A C-flat holey carbon grid with standard 20nm carbon thickness, 2.0 μm hole diameter, and 300 mesh Cu grid (CF-2/4-3Cu from Protochips Inc., North Carolina, USA) was hydrophilized by 2min of glow discharge using air and a remote source at 15W in a Tergeo EM Plasma Cleaner (PIE Scientific LLC, California, USA). ..

Article Title: Selective peptide bond formation via side chain reactivity and self-assembly of abiotic phosphates
Article Snippet: .. A C-flat holey carbon grid with standard 20 nm carbon thickness, 2.0 μm hole diameter, and 300 mesh Cu grid (CF-2/4-3Cu from Protochips Inc., North Carolina, USA) was hydrophilized by 2 min of glow discharge using air and a remote source at 15 W in a Tergeo EM Plasma Cleaner (PIE Scientific LLC, California, USA). ..

Article Title: Electrooxidation of 2‐Propanol on Mono‐ and Bi‐Metallic Noble Metal Nanoparticles in Alkaline Studied with Real‐Time Product and Dissolution Characterization
Article Snippet: .. Consecutively, the grids dried under air atmosphere and cleaned for 1 min with air plasma by using a Tergeo EM plasma cleaner (PIE Scientific). .. TEM micrographs were acquired using a Talos F200i (Thermo Fisher Scientific).

Article Title: Spontaneous base flipping helps drive Nsp15's preferences in double stranded RNA substrates.
Article Snippet: .. Cryo-EM sample preparation, data collection, and processing Within 24 h of SEC purification, catalytically-dead Nsp15 (H235A mutant) was diluted in a low-salt buffer (20mMHEPES pH 7.5, 100mM NaCl, 5mM MnCl2, 5mM DTT) to 0.75 μM and incubated with the unpaired U dsRNA in Fig. 2 (50 μM) at 4 °C for 2 h. Grids were prepared by sputtering C-flat R1.2/1.3 (Protochips) with a 30 nm thick layer of gold on the grid bar side using a Leica EM ACE-600 sputterer, then cleaned immediately before use with a Tergeo EM plasma cleaner (Pie Scientific) in immersionmodewith a power of 38W for a periodof 75 s. A Leica EMGP2 freezing robot with a sample application chamber held at 95% humidity and 20 °C equipped with Whatman Grade 40 filter paper was used to freeze grids: 4μL of sample was applied to the grid followedby a 5 swait time, 3 s backblot, andplunge freezing into liquid ethane. ..

Incubation:

Article Title: TFIIH kinase CDK7 drives cell proliferation through a common core transcription factor network.
Article Snippet: .. CAK- MAT1Δ219 (2 mg/ ml) was diluted 7× in the buffer containing the 54 μM SY- 5609 and incubated at room temperature for 5 min. SY- 1365 (purchased from Insight Biotech Ltd.) was dissolved in DMSO and diluted to 10 μM in cryo- EM buffer before formation of the covalent complex by incubation with diluted CAK complex for 15 min. For grid preparation, UltrAuFoil 1.2/1.3 300- mesh gold grids (Quantifoil Micro Tools GmbH) were plasma cleaned for 50 s using a Tergeo EM Plasma Cleaner (PIE Scientific). .. Four microliters of sample was applied on the grid and blotted for 1.5 s using a Vitrobot Mark IV (Thermo Fisher Scientific) and then plunge frozen in liquid ethane cooled by liquid nitrogen.

Article Title: Spontaneous base flipping helps drive Nsp15's preferences in double stranded RNA substrates.
Article Snippet: .. Cryo-EM sample preparation, data collection, and processing Within 24 h of SEC purification, catalytically-dead Nsp15 (H235A mutant) was diluted in a low-salt buffer (20mMHEPES pH 7.5, 100mM NaCl, 5mM MnCl2, 5mM DTT) to 0.75 μM and incubated with the unpaired U dsRNA in Fig. 2 (50 μM) at 4 °C for 2 h. Grids were prepared by sputtering C-flat R1.2/1.3 (Protochips) with a 30 nm thick layer of gold on the grid bar side using a Leica EM ACE-600 sputterer, then cleaned immediately before use with a Tergeo EM plasma cleaner (Pie Scientific) in immersionmodewith a power of 38W for a periodof 75 s. A Leica EMGP2 freezing robot with a sample application chamber held at 95% humidity and 20 °C equipped with Whatman Grade 40 filter paper was used to freeze grids: 4μL of sample was applied to the grid followedby a 5 swait time, 3 s backblot, andplunge freezing into liquid ethane. ..

Cryo-EM Sample Prep:

Article Title: TFIIH kinase CDK7 drives cell proliferation through a common core transcription factor network.
Article Snippet: .. CAK- MAT1Δ219 (2 mg/ ml) was diluted 7× in the buffer containing the 54 μM SY- 5609 and incubated at room temperature for 5 min. SY- 1365 (purchased from Insight Biotech Ltd.) was dissolved in DMSO and diluted to 10 μM in cryo- EM buffer before formation of the covalent complex by incubation with diluted CAK complex for 15 min. For grid preparation, UltrAuFoil 1.2/1.3 300- mesh gold grids (Quantifoil Micro Tools GmbH) were plasma cleaned for 50 s using a Tergeo EM Plasma Cleaner (PIE Scientific). .. Four microliters of sample was applied on the grid and blotted for 1.5 s using a Vitrobot Mark IV (Thermo Fisher Scientific) and then plunge frozen in liquid ethane cooled by liquid nitrogen.

Article Title: Spontaneous base flipping helps drive Nsp15's preferences in double stranded RNA substrates.
Article Snippet: .. Cryo-EM sample preparation, data collection, and processing Within 24 h of SEC purification, catalytically-dead Nsp15 (H235A mutant) was diluted in a low-salt buffer (20mMHEPES pH 7.5, 100mM NaCl, 5mM MnCl2, 5mM DTT) to 0.75 μM and incubated with the unpaired U dsRNA in Fig. 2 (50 μM) at 4 °C for 2 h. Grids were prepared by sputtering C-flat R1.2/1.3 (Protochips) with a 30 nm thick layer of gold on the grid bar side using a Leica EM ACE-600 sputterer, then cleaned immediately before use with a Tergeo EM plasma cleaner (Pie Scientific) in immersionmodewith a power of 38W for a periodof 75 s. A Leica EMGP2 freezing robot with a sample application chamber held at 95% humidity and 20 °C equipped with Whatman Grade 40 filter paper was used to freeze grids: 4μL of sample was applied to the grid followedby a 5 swait time, 3 s backblot, andplunge freezing into liquid ethane. ..

Sample Prep:

Article Title: Spontaneous base flipping helps drive Nsp15's preferences in double stranded RNA substrates.
Article Snippet: .. Cryo-EM sample preparation, data collection, and processing Within 24 h of SEC purification, catalytically-dead Nsp15 (H235A mutant) was diluted in a low-salt buffer (20mMHEPES pH 7.5, 100mM NaCl, 5mM MnCl2, 5mM DTT) to 0.75 μM and incubated with the unpaired U dsRNA in Fig. 2 (50 μM) at 4 °C for 2 h. Grids were prepared by sputtering C-flat R1.2/1.3 (Protochips) with a 30 nm thick layer of gold on the grid bar side using a Leica EM ACE-600 sputterer, then cleaned immediately before use with a Tergeo EM plasma cleaner (Pie Scientific) in immersionmodewith a power of 38W for a periodof 75 s. A Leica EMGP2 freezing robot with a sample application chamber held at 95% humidity and 20 °C equipped with Whatman Grade 40 filter paper was used to freeze grids: 4μL of sample was applied to the grid followedby a 5 swait time, 3 s backblot, andplunge freezing into liquid ethane. ..

Purification:

Article Title: Spontaneous base flipping helps drive Nsp15's preferences in double stranded RNA substrates.
Article Snippet: .. Cryo-EM sample preparation, data collection, and processing Within 24 h of SEC purification, catalytically-dead Nsp15 (H235A mutant) was diluted in a low-salt buffer (20mMHEPES pH 7.5, 100mM NaCl, 5mM MnCl2, 5mM DTT) to 0.75 μM and incubated with the unpaired U dsRNA in Fig. 2 (50 μM) at 4 °C for 2 h. Grids were prepared by sputtering C-flat R1.2/1.3 (Protochips) with a 30 nm thick layer of gold on the grid bar side using a Leica EM ACE-600 sputterer, then cleaned immediately before use with a Tergeo EM plasma cleaner (Pie Scientific) in immersionmodewith a power of 38W for a periodof 75 s. A Leica EMGP2 freezing robot with a sample application chamber held at 95% humidity and 20 °C equipped with Whatman Grade 40 filter paper was used to freeze grids: 4μL of sample was applied to the grid followedby a 5 swait time, 3 s backblot, andplunge freezing into liquid ethane. ..

Mutagenesis:

Article Title: Spontaneous base flipping helps drive Nsp15's preferences in double stranded RNA substrates.
Article Snippet: .. Cryo-EM sample preparation, data collection, and processing Within 24 h of SEC purification, catalytically-dead Nsp15 (H235A mutant) was diluted in a low-salt buffer (20mMHEPES pH 7.5, 100mM NaCl, 5mM MnCl2, 5mM DTT) to 0.75 μM and incubated with the unpaired U dsRNA in Fig. 2 (50 μM) at 4 °C for 2 h. Grids were prepared by sputtering C-flat R1.2/1.3 (Protochips) with a 30 nm thick layer of gold on the grid bar side using a Leica EM ACE-600 sputterer, then cleaned immediately before use with a Tergeo EM plasma cleaner (Pie Scientific) in immersionmodewith a power of 38W for a periodof 75 s. A Leica EMGP2 freezing robot with a sample application chamber held at 95% humidity and 20 °C equipped with Whatman Grade 40 filter paper was used to freeze grids: 4μL of sample was applied to the grid followedby a 5 swait time, 3 s backblot, andplunge freezing into liquid ethane. ..



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